Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis

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Standard

Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis. / Skogstrand, Kristin; Thysen, Anna H.; Jørgensen, Charlotte S.; Rasmussen, E. Michael; Andersen, Åse B.; Lillebaek, Troels; Hougaard, David M.; Houen, Gunnar.

In: Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 72, No. 3, 05.2012, p. 204-211.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Skogstrand, K, Thysen, AH, Jørgensen, CS, Rasmussen, EM, Andersen, ÅB, Lillebaek, T, Hougaard, DM & Houen, G 2012, 'Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis', Scandinavian Journal of Clinical and Laboratory Investigation, vol. 72, no. 3, pp. 204-211. https://doi.org/10.3109/00365513.2011.649014

APA

Skogstrand, K., Thysen, A. H., Jørgensen, C. S., Rasmussen, E. M., Andersen, Å. B., Lillebaek, T., Hougaard, D. M., & Houen, G. (2012). Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis. Scandinavian Journal of Clinical and Laboratory Investigation, 72(3), 204-211. https://doi.org/10.3109/00365513.2011.649014

Vancouver

Skogstrand K, Thysen AH, Jørgensen CS, Rasmussen EM, Andersen ÅB, Lillebaek T et al. Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis. Scandinavian Journal of Clinical and Laboratory Investigation. 2012 May;72(3):204-211. https://doi.org/10.3109/00365513.2011.649014

Author

Skogstrand, Kristin ; Thysen, Anna H. ; Jørgensen, Charlotte S. ; Rasmussen, E. Michael ; Andersen, Åse B. ; Lillebaek, Troels ; Hougaard, David M. ; Houen, Gunnar. / Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis. In: Scandinavian Journal of Clinical and Laboratory Investigation. 2012 ; Vol. 72, No. 3. pp. 204-211.

Bibtex

@article{9dcd5f1c1aa0442a845e7f3c4287979c,
title = "Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis",
abstract = "Background: In vitro stimulation of whole blood or isolated blood cells with specific antigens is used for several purposes. Immediately following incubation with antigens, samples have to be centrifuged to stop the reactions by remaining cells and the supernatant refrigerated or analysed directly to preserve the analytes of interest, which makes samples difficult to prepare outside laboratories. We have tested whether spotting whole blood on filter paper after activation can be used in one of the tests for Mycobacterium tuberculosis infection (MTI), the QuantiFERON{\textregistered}-TB Gold In Tube test (QFT), where the spotting technique can make it suitable for use in locations without facilities like a centrifuge and a refrigerator. Materials and methods: Samples from 22 individuals undergoing screening for MTI and 10 healthy controls were incubated, centrifuged and IFN-γ measured by Enzyme-linked immunosorbent assay (ELISA), as described in the kit insert. In parallel, activated blood was spotted on filter paper (Schleicher & Schuell) and dried. The dried blood spot samples were analysed for 21 inflammatory markers with an in-house assay based on Luminex technology. Results: Our multiplex measurements of inflammatory markers in samples from suspected MTI patients confirmed the IFN-γ findings in the QFT. IL-2, GM-CSF, IL-5, and IL-1β were also found as useful markers for MTI. We were not able to distinguish between active tuberculosis and latent MTI. Conclusion: Applying blood on filter paper after incubation makes in vitro stimulation tests feasible in locations where heat and electricity is unavailable.",
keywords = "Antigens, Cytokines, Immunologic stimulation, In-vitro, Mycobacterium tuberculosis",
author = "Kristin Skogstrand and Thysen, {Anna H.} and J{\o}rgensen, {Charlotte S.} and Rasmussen, {E. Michael} and Andersen, {{\AA}se B.} and Troels Lillebaek and Hougaard, {David M.} and Gunnar Houen",
year = "2012",
month = may,
doi = "10.3109/00365513.2011.649014",
language = "English",
volume = "72",
pages = "204--211",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation. Supplement",
issn = "0085-591X",
publisher = "Taylor & Francis",
number = "3",

}

RIS

TY - JOUR

T1 - Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis

AU - Skogstrand, Kristin

AU - Thysen, Anna H.

AU - Jørgensen, Charlotte S.

AU - Rasmussen, E. Michael

AU - Andersen, Åse B.

AU - Lillebaek, Troels

AU - Hougaard, David M.

AU - Houen, Gunnar

PY - 2012/5

Y1 - 2012/5

N2 - Background: In vitro stimulation of whole blood or isolated blood cells with specific antigens is used for several purposes. Immediately following incubation with antigens, samples have to be centrifuged to stop the reactions by remaining cells and the supernatant refrigerated or analysed directly to preserve the analytes of interest, which makes samples difficult to prepare outside laboratories. We have tested whether spotting whole blood on filter paper after activation can be used in one of the tests for Mycobacterium tuberculosis infection (MTI), the QuantiFERON®-TB Gold In Tube test (QFT), where the spotting technique can make it suitable for use in locations without facilities like a centrifuge and a refrigerator. Materials and methods: Samples from 22 individuals undergoing screening for MTI and 10 healthy controls were incubated, centrifuged and IFN-γ measured by Enzyme-linked immunosorbent assay (ELISA), as described in the kit insert. In parallel, activated blood was spotted on filter paper (Schleicher & Schuell) and dried. The dried blood spot samples were analysed for 21 inflammatory markers with an in-house assay based on Luminex technology. Results: Our multiplex measurements of inflammatory markers in samples from suspected MTI patients confirmed the IFN-γ findings in the QFT. IL-2, GM-CSF, IL-5, and IL-1β were also found as useful markers for MTI. We were not able to distinguish between active tuberculosis and latent MTI. Conclusion: Applying blood on filter paper after incubation makes in vitro stimulation tests feasible in locations where heat and electricity is unavailable.

AB - Background: In vitro stimulation of whole blood or isolated blood cells with specific antigens is used for several purposes. Immediately following incubation with antigens, samples have to be centrifuged to stop the reactions by remaining cells and the supernatant refrigerated or analysed directly to preserve the analytes of interest, which makes samples difficult to prepare outside laboratories. We have tested whether spotting whole blood on filter paper after activation can be used in one of the tests for Mycobacterium tuberculosis infection (MTI), the QuantiFERON®-TB Gold In Tube test (QFT), where the spotting technique can make it suitable for use in locations without facilities like a centrifuge and a refrigerator. Materials and methods: Samples from 22 individuals undergoing screening for MTI and 10 healthy controls were incubated, centrifuged and IFN-γ measured by Enzyme-linked immunosorbent assay (ELISA), as described in the kit insert. In parallel, activated blood was spotted on filter paper (Schleicher & Schuell) and dried. The dried blood spot samples were analysed for 21 inflammatory markers with an in-house assay based on Luminex technology. Results: Our multiplex measurements of inflammatory markers in samples from suspected MTI patients confirmed the IFN-γ findings in the QFT. IL-2, GM-CSF, IL-5, and IL-1β were also found as useful markers for MTI. We were not able to distinguish between active tuberculosis and latent MTI. Conclusion: Applying blood on filter paper after incubation makes in vitro stimulation tests feasible in locations where heat and electricity is unavailable.

KW - Antigens

KW - Cytokines

KW - Immunologic stimulation

KW - In-vitro

KW - Mycobacterium tuberculosis

U2 - 10.3109/00365513.2011.649014

DO - 10.3109/00365513.2011.649014

M3 - Journal article

C2 - 22283828

AN - SCOPUS:84859599026

VL - 72

SP - 204

EP - 211

JO - Scandinavian Journal of Clinical and Laboratory Investigation. Supplement

JF - Scandinavian Journal of Clinical and Laboratory Investigation. Supplement

SN - 0085-591X

IS - 3

ER -

ID: 247164404